Abstract
Advancements in life sciences increasingly demand imaging systems capable of combining high spatial resolution, fast acquisition, and compatibility with thick or live samples. While individual fluorescence microscopy techniques each offer specific strengths, they are often limited in their ability to meet all these needs simultaneously. Correlative multimodal imaging addresses this challenge by integrating complementary technologies to provide comprehensive insights into complex biological systems. Here, we present the X-Light V3 + DeepSIM system by CrestOptics, a versatile platform that combines spinning disk confocal microscopy with lattice structured illumination microscopy. This integrated system enables rapid, large-field imaging in confocal mode, seamlessly transitioning to super-resolution mode for detailed visualization of subcellular structures, all using shared optics and standard sample preparation. We demonstrate its capabilities in neurobiology by imaging dendritic spine morphology in brain slices. The system’s high-resolution performance allows accurate morphological measurements—such as head-to-neck ratios in dendritic spines—critical for studying synaptic plasticity and disease. These results underscore the power of correlative multimodal imaging to accelerate and enhance biomedical research.
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