June 3-6, 2025
Join us at ELMI conference 2025!
CrestOptics is thrilled to announce our participation in the European Light Microscopy Initiative (ELMI) 2025, from June 3 to 6, 2025, at EMBL Heidelberg, Germany. This exciting event is a prime opportunity to connect with the dynamic microscopy community, including European scientists, equipment manufacturers, and industry leaders, all working together to push the boundaries of light microscopy in life sciences research.
Discover Our Cutting-Edge Microscopy Solutions
Visit us at booth F28 to explore our advanced spinning disk and super-resolution systems, designed to enable researchers to capture high-quality, detailed images and enhance the visualization of complex biological processes.
Don’t Miss Our Workshops!
Our experts will be hosting engaging and educational workshops in Microscopy Room 117a at the ATC (Advanced Training Center). We are excited to offer attendees exclusive insights into the latest microscopy advancements through hands-on, interactive sessions. The microscopy setup featured during the workshops includes a Ti2 Eclipse (Nikon) combined with our X-Light V3 spinning disk and DeepSIM super-resolution systems, along with a Kinetix camera (Photometrics) and LDI-4 laser 405/470/555/640 nm (89North). Don’t miss the chance to deepen your expertise and connect with our specialists!
To secure your spot, click the link below and register for your preferred session.
Participants are encouraged to bring their own samples for personalized, real-time demos.
Spaces are limited, and the deadline for registration is May 21, 2025.
Click the link below to book your individual imaging session with CrestOptics’ X-Light V3 Spinning Disk and DeepSIM X-Light Super-resolution technologies.
A Sneak Peek at Our Workshops Below!
1) Unlocking 3D Complexity: Integrated Confocal and Super-Resolution Microscopy for Organoid and Model Organism Analysis
Read the workshop abstract
Abstract: The study of complex biological systems, such as organoids and model organisms, demands imaging techniques that can capture both large-scale architecture and subcellular details in three dimensions. This workshop presents a powerful approach combining CrestOptics’ X-Light V3 spinning disk confocal microscopy for rapid volumetric imaging with DeepSIM super-resolution microscopy for nanoscale resolution. The X-Light V3 enables efficient acquisition of large 3D datasets, while DeepSIM provides the necessary super-resolution to resolve intricate structures within these models. We will demonstrate the capabilities of this integrated workflow for high-content analysis of organoids and whole-organism imaging, highlighting its potential to accelerate discoveries in developmental biology, disease modeling, and drug discovery.
2) Lattice SIM across scales: breaking the diffraction limit barrier in Z-depth
Read the workshop abstract
Abstract: Deep tissue imaging in complex biological samples is often limited by diffraction and scattering, particularly hindering the visualization of subcellular structures in the axial (Z) dimension. This workshop demonstrates the application of CrestOptics’ DeepSIM, a lattice-based structured illumination microscopy technique, to overcome these limitations and achieve enhanced Z-depth resolution across diverse biological scales. We will present data showcasing DeepSIM’s capabilities in resolving intricate subcellular details in HeLa cells, visualizing deep cellular architecture in cleared mouse brain tissue, and mapping the complex morphology and interactions of astrocytes. The results highlight DeepSIM’s potential for providing new insights into fundamental biological processes, from cellular dynamics to tissue organization, across various model systems.
3) Multiplexed Confocal Imaging: A High-Throughput Approach to Deciphering Spatial Omics
Imaging on: Whole Brain Slice samples
Read the workshop abstract
Abstract: Understanding the spatial relationships between various molecular markers in complex tissues, such as the whole brain, is fundamental to unraveling the principles of brain function and disease. This workshop will showcase a multiplexed imaging strategy employing CrestOptics’ X-Light V3 and DeepSIM for high-content analysis of whole brain slices. X-Light V3 facilitates rapid acquisition of large volumetric datasets, enabling the simultaneous visualization of multiple markers across the entire tissue. DeepSIM is then used to resolve subcellular details within these multiplexed datasets, providing crucial information on protein localization and interactions. This workflow offers a powerful tool for spatial omics studies, linking molecular identity to cellular and tissue context.
