X-Light V2
UNIVERSAL PLUG-IN SPINNING DISK
FOR FAST AND GENTLE IMAGING
Full compatibility
Plug-in spinning disk
Custom relay lenses
Wider field of view
Description
To guarantee each laboratory greater productivity without compromising the data quality, CrestOptics has created the X-Light V2, the most accessible spinning disk solution for fast and gentle confocal imaging.
The X-light V2 is a truly enabling technology where the high-performance is combined with the application fexibility and a universal compatibility with any upright and inverted microscopes with a camera port.
Heart Cell – Isolated myocyte imaged at 330 fps, calcium signal on the right
25mm field of view
The X-light V2 enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.
The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the X- light V2 the standard tool for 3D live cell imaging.
Visit our Publications page to explore a collection of scientific articles, where our cutting-edge microscopy instruments have been utilized, enabling significant discoveries across various research fields
Image Gallery
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Technical Features
| Imaging modalities | Widefield/Confocal |
| Compatible microscopes | Full range of upright a Compatible microscopes nd inverted microscopes |
| FOV | Up to 25 mm diagonal |
| Light source | LASER with 1,5 mm SMA | LED with 3 mm LLG |
| Spectral range | Excitation 400-750 nm / Emission 400-850 nm |
| Camera | Compatible with CCD/EMCCD cameras and sCMOS cameras |
| Disk speed/scan rate | 15000 RPM / > 1000 fps |
| Spinning disk geometry (diameter/spacing) | 50/250 μm pinholes optimized for LASER light source 60/220 μm pinholes optimized for LED light source Slit spirals for high throughput & live imaging applications Customized disk available upon request |
| Resolution | Lateral Resolution (FWHM): ~230 nm (High NA 1.4) Axial Resolution (FWHM): ~600 nm (High NA 1.4) |
| Filter wheels | Motorized filter wheels: 8-positions excitation filter wheel, 5-positions dichroic filter wheel, 8-positions emission filter wheel. |
| Software | NIS Elements / μManager / MetaMorph/ Volocity / VisiView® |
| Weight | 11.0 Kg (24.3 lbs) |
| Dimensions | 270 (w) x 325 (l) x 226.5 (h) mm 10.6 (w) x 12.8 (l) x 8.9 (h) inches |
Compatibility
List of compatible products
Detectors
Microscopes
Nikon
Evident (former Olympus)
Leica
Zeiss
Illuminators
Lumencor – SPECTRA light engine
Lumencor – CELESTA light engine
CoolLED pE800
Excelitas Novem
Software
MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView
Inscoper
Neurolucida
Stereoinvestigator
For more information
Commercial Partners
CrestOptics relies on a worldwide network of experienced and trained commercial partners who provide full commercial and technical support to laboratories and facilities
