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Spinning disk confocal revolutionising the visualization of biologic sample all at once
Dual-Camera Ultra-Large Field of View
Uniform Illumination
Ultrafast
Sealed
Disk
Upgradable
to
Super-Resolution
At CrestOptics, we believe that spinning disk confocal technology should be the workhorse for life science research. It has to effectively address the majority of contemporary fluorescence microscopy applications.
The X-Light V3 relies on cutting-edge technology to provide precise 3D sectioning at the highest speed and largest field of view maintaining uniform illumination. Highly flexible in configuration, it provides the possibility to interchange the disk box to image conventional and complex samples.
Do you want to know more about X-Light V3 technology?
Do you want to see the X-Light V3 in action?
Don't Just Image It. DeepSIM It.
Take your X-Light V3 Spinning Disk to the next level with DeepSIM Super Resolution system. Contact us to schedule a remote DeepSIM demo and learn more about our special offer, valid through the end of 2024!
X-Light V3 at a glance
Dr. Gaia Pigino Human Technopole (Milan)
Dr. Gabriela ImrehKarolinska Institutet (Stockholm)
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Description
Ultra-large field of view (FOV) up to 25 mm
The first confocal system with a 25 mm FOV on single and dual cameras. The ultra-large FOV allows the capture of more data or a full organism in a single frame and reduces the number of tiles necessary to cover very large specimens, speeding up research discoveries.
HeLa cells stained with DAPI for DNA (blue), tubulin (green), mitochondria (orange), nucleoli (red). The traditional Spinning Disk (SD) confocal FOV (18
mm) is shown (white dotted box) compared with the large (25 mm) X-Light V3 FOV (yellow box). 60x Oil, NA 1.42.
Uniform illumination over the entire FOV
Retrieving uniform signal is essential for accurate quantitative imaging and seamless stitching of large samples, thus generating robust datasets. Thanks to the use of a microlens array placed directly inside the illuminator, the X-Light V3 provides homogeneous illumination over the entire 25 mm FOV.
(i) Human cerebral organoid (day 50) showing CTIP2-positive deep layer cortical neurons (white), pan-neuronal MAP2 marker signal (red), and nuclei stained with DAPI (blue). A large stitched image is shown. 20x Air, NA 0.75. Sample credits: Prof. Di Angelantonio, CLN2S@Sapienza Università di Roma – IIT. (ii) Mouse kidney tissue section imaging: a comparison between the traditional illuminator (left), which introduces vignetting artifacts, and the
X-Light V3 Micro-lenses illuminator (right), providing accurate, artifact-free data without the need for post-processing corrections.
Revolutionise your imaging
Ready to accept a second camera, the X-Light V3 goes beyond standard imaging. With the capability of hosting two equal cameras to perform fast dual-colour simultaneous imaging or two different cameras for different applications, it provides the highest flexibility to meet an extensive variety of imaging requirements. Still with a 25 mm FOV.
3D movie of AD-MSCs TRAIL cells (GFP-green) actively migrating inside the VITVO bioreactor matrix (by Rigenerand) and surrounding A673 tumor cells (drRED -red), till to form apoptotic bodies before death. A total Z volume of 450 um was acquired. 4x Air, NA 0.2.
Highest spinning disk rotation for fast imaging
Speed is essential to carry out advanced imaging experiments efficiently. The X-Light V3 offers an achievable acquisition speed of over 1000 fps thanks to the highest disk speed rotation in the market (15.000 rpm) and synchronization to the camera as a default feature.
Live acquisition of LLC-PK1 cells expressing EB3-mEmerald (green) and H2B-mCherry (red). A mitotic division is shown. 63x Oil, NA 1.4. Sample credits: 89 North distributor.
Disk box – sealed, motorized, tailored
Excellent imaging comes from excellent design: the X-Light V3 disk box has unique features to ensure high-end performance in every circumstance.
– Fully sealed: isolation and protection from environmental dust are essential to prevent undesired artefacts due to the presence of dust particles on disk surface.
– Motorized disk box: move between widefield and confocal imaging with a simple click from the software.
– Tailored disk geometry specifically designed for key applications.
Large 3D volumetric view of a cleared brain organoid. In-depth color coding of a total Z volume of 250 um showing MAP2 staining. 20x Air, NA 0.8. Sample credits: Dr. Debbi and Prof. Di Angelantonio, CLN2S@Sapienza Università di Roma – IIT.
Illumination shaping
A software-controlled square iris can be used to illuminate only a small portion of the FOV making the X-Light V3 suitable for photo-activation and bleaching experiments.
Hela cells showing tubulin (green) and DNA (blue). (i) 100% of the entire FOV. (ii) 25% of the entire FOV. 60x Oil, 1.4 NA.
Click here to see how to modify the iris during live acquisition.
Broad fluorophores choice
The X-Light V3 offers the maximum flexibility in fluorophore choice, providing a broad wavelength range from 405 nm to 750 nm excitation. Many applications benefit from this, such as spatial transcriptomics and multiplexed imaging that gather information from multiple cellular markers.
(i) Large image of a mouse brain section marked with different 4 different RNA species; nuclei are stained in blue for in situ mapping of neuronal subtypes. (ii) Close-up of image (i): single spots and RNA molecules localization in the hippocampal region. 40x water, NA 1.15. Sample credits: Dr. Kwasi Kwakwa and Dr. Omer Bayraktar, Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Upgradable to Super-Resolution on the same set-up
The X-Light V3 can be effortlessly upgraded to super-resolution by integrating DeepSIM, allowing researchers to seamlessly enhance their imaging capabilities from widefield to confocal to super-resolution.
Hippocampal coronal slice from Thyl-GFP mouse brain shown in three modes of use (Widefield – WF, Spinning disk confocal – CF, Super-resolution – SR). 20x Air, NA 0.75.
Application focus
Visit our Publications page to explore a collection of scientific articles, where our cutting-edge microscopy instruments have been utilized, enabling significant discoveries across various research fields
Evolution through compatibility
X-Light V3 is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. X-Light V3 can seamlessly upgrade any existing frame with a camera port, be it upright or inverted, thus leading to a significant cost-saving setup.
In addition, the X-Light V3 can be smoothly upgraded to super-resolution by adding DeepSIM, evolving every researcher’s imaging capabilities from widefield to confocal to super-resolution with ease.
Technical features
| Imaging modalities | Widefield/Confocal/Super-resolution in combination with DeepSIM X-Light |
| Compatible microscopes | Full range upright and inverted microscopes |
| FOV | Up to 25 mm diagonal |
| Laser | Multi mode lasers with SMA coupling |
| Spectral range | Excitation 400-750 nm/ Emission 400-850 nm |
| Camera | Compatible with CCD/EMCCD cameras and sCMOS cameras Dual-camera view: simoultaneous dual-camera imaging up to 25 mm |
| Disk speed/scan rate | 15000 RPM / > 1000 fps |
| Spinning disk geometry | Pinholes and slit spirals: different geometries to match with key applications. |
| Resolution | Lateral Resolution (FWHM): ~230 nm (High NA 1,4) Axial Resolution (FWHM): ~600 nm (High NA 1,4) |
| Filter wheels | Motorized filter wheels and slider: 4-positions excitation filter wheel, 3-positions dichroic filter wheel, 8-positions emission filter wheel, 3 positions slider for dual camera use |
| Software | NIS Elements / μManager / Volocity / VisiView® |
| Recommended installation conditions | Temperature 23± 5°C, Humidity 70% RH or less (no condensation) |
| Weight | 26,0 Kg (57 lbs) |
| Dimensions | X-Light V3: 370 (w) x 555.6 (l) x 226.5 (h) mm 14.5 (w) x 21.9 (l) x 8.9 (h) inches X-Light V3 + DeepSIM X-Light: |
The product packaging has been certified by the International Safe Transit Association (ISTA).
Compatibility
List of compatible products
Detectors
Hamamatsu
Microscopes
Evident (former Olympus)
Leica
Zeiss
Illuminators
Lumencor – CELESTA light engine
Software
MicroManager
Volocity
VisiView
For more information
Commercial Partners
CrestOptics relies on a worldwide network of experienced and trained commercial partners who provide full commercial and technical support to laboratories and facilities
